Article
Distance variations between active sites of H(+)-pyrophosphatase determined by fluorescence resonance energy transfer.
The Journal of biological chemistry - 30 Jul 2010
Huang Yun-Tzu, Liu Tseng-Huang, Chen Yen-Wei, Lee Chien-Hsien, Chen Hsueh-Hua, Huang Tsu-Wei, Hsu Shen-Hsing, Lin Shih-Ming, Pan Yih-Jiuan, Lee Ching-Hung, Hsu Ian C, Tseng Fan-Gang, Fu Chien-Chung, Pan Rong-Long
Abstract excerpt
Homodimeric H(+)-pyrophosphatase (H(+)-PPase; EC 3.6.1.1) is a unique enzyme playing a pivotal physiological role in pH homeostasis of organisms. This novel H(+)-PPase supplies energy at the expense of hydrolyzing metabolic byproduct, pyrophosphate (PP(i)), for H(+) translocation across membrane. The functional unit for the translocation is considered to be a homodimer. Its putative active site on each subunit...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.