Article
Properties of mutated Rhodospirillum rubrum H+-pyrophosphatase expressed in Escherichia coli.
Biochimica et biophysica acta - 8 Dec 2003
Schultz Anders, Baltscheffsky Margareta
Abstract excerpt
The membrane-bound proton pumping inorganic pyrophosphate synthase/pyrophosphatase (H(+)-PPi synthase/H(+)-PPase) from the photosynthetic bacterium Rhodospirillum rubrum was functionally expressed in Escherichia coli C43(DE3) cells. Based on a new topology model of the enzyme, charged residues pr...
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