Article
Sites of preferential induction of cyclobutane pyrimidine dimers in the nontranscribed strand of lacI correspond with sites of UV-induced mutation in Escherichia coli.
The Journal of biological chemistry - 25 Jun 1991
Koehler D R, Awadallah S S, Glickman B W
Abstract excerpt
An approach utilizing fluorescence-activated DNA sequencing technology was used to study the position and frequency of UV-induced lesions in the lacI gene of Escherichia coli. The spectrum of sites of UV damage in the NC+ region of the gene was compared with a published spectrum of UV-induced mutation in lacI (Schaaper, R.M., Dunn, R.L., and Glickman, B.W. (1987) J. Mol. Biol. 198, 187-202). On average, the...
Topics
- Escherichia coli
- Fluorescence
- Genes, Bacterial
- Mutation
- Pyrimidine Dimers
- Spectrophotometry, Ultraviolet
- Ultraviolet Rays