Article
Signal peptidase and oligosaccharyltransferase interact in a sequential and dependent manner within the endoplasmic reticulum.
The Journal of biological chemistry - 26 Jan 2001
Chen X, VanValkenburgh C, Liang H, Fang H, Green N
Abstract excerpt
We demonstrate that the signal peptides of prepro-alpha-factor and preinvertase must be cleaved before Asn-X-Ser/Thr acceptor tripeptides located near the signal peptides of these precursors can be efficiently glycosylated within the endoplasmic reticulum of the yeast Saccharomyces cerevisiae. The data support a model whereby the interaction of a signal peptide with the membrane prevents an acceptor tripeptide...
Topics
- Amino Acid Sequence
- Endoplasmic Reticulum
- Enzyme Precursors
- Fungal Proteins
- Glycoside Hydrolases
- Glycosylation
- Hexosyltransferases
- Membrane Proteins
- Models, Biological
- Molecular Sequence Data
- Mutation
- Peptide Hydrolases
- Protein Precursors