Article
Purification and characterization of recombinant tropomyosins.
Journal of chromatography - 22 Feb 1991
Ferraz C, Sri Widada J, Liautard J P
Abstract excerpt
The cloning of a cDNA coding for the skeletal human beta-tropomyosin in the bacterial expression vector pKK233-2 is reported. Deletion mutants were also constructed. pCF-T1088 was obtained by elimination of exon 9 and pCF-T1089 was built by deleting 2/3 of the first exon. The recombinant tropomyo...
Topics
- Amino Acid Sequence
- Chromatography, High Pressure Liquid
- Chromosome Deletion
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Ethanolamines
- Humans
- Hydroxyapatites
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Tropomyosin
- Trypsin