Article
Construction and cloning of recombinant rat trypstatin variants and their expression as fusion proteins in E. coli.
Biomedica biochimica acta - 1 Jan 1991
Dolinar M, Auerswald E A
Abstract excerpt
A synthetic master gene coding for rat trypstatin was designed, assembled of eight oligonucleotides, ligated into the cloning vector pUC8 and cloned in E. coli. In addition to the expected product DNA sequencing revealed the presence of several gene variants. The gene coding for (-1M F44G) rat trypstatin was mutated to a wild type form (-1M) rat trypstatin by cassette mutagenesis. For the first expression...
Topics
- Alpha-Globulins
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA
- Escherichia coli
- Genetic Variation
- Genetic Vectors
- Molecular Sequence Data
- Proteins