Article
Construction, expression and unexpected regulatory properties of a tropomyosin mutant with a 31-residue deletion at the C-terminus (exon 9).
European journal of biochemistry - 27 Dec 1990
Bartegi A, Ferraz C, Fattoum A, Sri Widada J, Heitz F, Kassab R, Liautard J P
Abstract excerpt
The cDNA coding for human skeletal muscle beta-tropomyosin was expressed in Escherichia coli to produce an unacetylated beta-tropomyosin. This cDNA was deleted from the sequence corresponding to the exon 9 and expressed in E. coli to produce an unacetylated beta-tropomyosin mutant lacking the C-t...
Topics
- Actins
- Adenosine Triphosphatases
- Animals
- Base Sequence
- Chromosome Deletion
- DNA
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Escherichia coli
- Exons
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Humans
- Molecular Sequence Data
- Muscles
- Mutation
- Osmolar Concentration
- Rabbits