Article
Fluorescence properties and functional roles of tryptophan residues 60d, 96, 148, 207, and 215 of thrombin.
The Journal of biological chemistry - 22 Sept 2000
Bell R, Stevens W K, Jia Z, Samis J, Côté H C, MacGillivray R T, Nesheim M E
Abstract excerpt
Conservative Trp-to-Phe mutations were individually created in human thrombin at positions 60d, 96, 148, 207, and 215. Fluorescence intensities for these residues varied by a factor of 6. Residues 60d, 96, 148, and 215 transferred energy to the thrombin inhibitor 5-dimethylaminonaphthalene-1-sulfonylarginine-N-(3-ethyl-1,5- pentanediyl)amide efficiently, but residue 207 did not. Intensities correlated inversely...
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