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Article

Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

Biochemical and biophysical research communications - 14 May 2010

Takahashi Hitoshi, Akazawa Daisuke, Kato Takanobu, Date Tomoko, Shirakura Masayuki, Nakamura Noriko, Mochizuki Hidenori, Tanaka-Kaneko Keiko, Sata Tetsutaro, Tanaka Yasuhito, Mizokami Masashi, Suzuki Tetsuro, Wakita Takaji

Abstract excerpt

To establish a simple system for purification of recombinant infectious hepatitis C virus (HCV) particles, we designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutation at the potential N-glycosylation site (E2N151K) leads to efficient production of the chimeric...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 20399750
DOI: 10.1016/j.bbrc.2010.04.081

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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