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Article

Biochemical and structural characterization of two site-directed mutants of Staphylococcus xylosus lipase.

Molecular biotechnology - 1 Oct 2010

Kolling Deise Juliana, Bertoldo Jean Borges, Brod Fábio Cristiano Angonesi, Vernal Javier, Terenzi Hernán, Arisi Ana Carolina Maisonnave

Abstract excerpt

Staphylococcus xylosus AF208229 lipase was expressed in E. coli containing an histidine-tag (WT-Val). In the present work, in order to check the importance of the residue 309 in the specific activity, the amino acid side chain residue valine 309 was substituted by aspartate or lysine through site-directed mutagenesis. Both mutant lipases (MUT-Lys and MUT-Asp) were expressed in E. coli and the recombinant...

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PMID: 20387014
DOI: 10.1007/s12033-010-9282-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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