Article
Expression of a dominant negative allele of cdc2 prevents activation of the endogenous p34cdc2 kinase.
Molecular & general genetics : MGG - 1 May 1991
Fleig U N, Nurse P
Abstract excerpt
The cdc2 gene of the fission yeast Schizosaccharomyces pombe encodes a 34 kDa phosphoprotein with serine/threonine protein kinase activity that acts as the key component in regulation of the eukaryotic cell cycle. We used a repressible promoter fused to the cdc2 cDNA to isolate conditionally dominant negative mutants of cdc2. One of these mutants, DL5, is described in this paper. Overexpression of the mutant...
Topics
- Alleles
- Amino Acid Sequence
- Blotting, Western
- CDC2 Protein Kinase
- Enzyme Activation
- Genes, Dominant
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- Precipitin Tests