Article
Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid.
The Journal of biological chemistry - 5 Jun 1991
Jenal U, Rechsteiner T, Tan P Y, Bühlmann E, Meile L, Leisinger T
Abstract excerpt
The ileS gene encoding the isoleucyl-tRNA synthetase of the thermophilic archaebacterium Methanobacterium thermoautotrophicum Marburg was isolated and sequenced. ileS was closely flanked by an unknown open reading frame and by purL and thus is arranged differently from the organizations observed...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Euryarchaeota
- Isoleucine-tRNA Ligase
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Plasmids
- Restriction Mapping
- Sequence Alignment