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Article

Genetic engineering of a sake yeast producing no urea by successive disruption of arginase gene.

Applied and environmental microbiology - 1 Jan 1991

Kitamoto K, Oda K, Gomi K, Takahashi K

Abstract excerpt

Urea is reported to be a main precursor of ethyl carbamate (ECA), which is suspected to be a carcinogen, in wine and sake. In order to minimize production of urea, arginase-deficient mutants (delta car1/delta car1) were constructed from a diploid sake yeast, Kyokai no. 9, by successive disruption of the two copies of the CAR1 gene. First, the yeast strain was transformed with plasmid pCAT2 (delta car1 SMR1), and...

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Identifiers

PMID: 2036017
DOI: 10.1128/aem.57.1.301-306.1991
PMCID: PMC182702

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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