Genetic engineering of a sake yeast producing no urea by successive disruption of arginase gene.

doi:10.1128/aem.57.1.301-306.1991

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Article

Genetic engineering of a sake yeast producing no urea by successive disruption of arginase gene.

Applied and environmental microbiology - 1 Jan 1991

Kitamoto K, Oda K, Gomi K, Takahashi K

Abstract excerpt

Urea is reported to be a main precursor of ethyl carbamate (ECA), which is suspected to be a carcinogen, in wine and sake. In order to minimize production of urea, arginase-deficient mutants (delta car1/delta car1) were constructed from a diploid sake yeast, Kyokai no. 9, by successive disruption...

Topics

Alcoholic Beverages
Arginase
Food Microbiology
Genes, Fungal
Genetic Engineering
Mutation
Saccharomyces cerevisiae
Urea
Urethane

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