Article
Normal targeting of a tagged Kv1.5 channel acutely transfected into fresh adult cardiac myocytes by a biolistic method.
American journal of physiology. Cell physiology - 1 Jun 2010
Dou Ying, Balse Elise, Dehghani Zadeh Alireza, Wang Tiantian, Goonasekara Charitha L, Noble Geoffrey P, Eldstrom Jodene, Steele David F, Hatem Stéphane N, Fedida David
Abstract excerpt
The transfection of cardiac myocytes is difficult, and so most of the data regarding the regulation of trafficking and targeting of cardiac ion channels have been obtained using heterologous expression systems. Here we apply the fast biolistic transfection procedure to adult cardiomyocytes to show that biolistically introduced exogenous voltage-gated potassium channel, Kv1.5, is functional and, like endogenous...
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