Article
Tentacle Probes: differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time PCR.
Clinical chemistry - 1 Dec 2007
Satterfield Brent C, Kulesh David A, Norwood David A, Wasieloski Leonard P, Caplan Michael R, West Jay A A
Abstract excerpt
BACKGROUND: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation. METHODS: Tentacle Probes, cooperative reagents with both a capture and a...
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