Article
Effect of uracil situated in the vicinity of a mispair on the directionality of mismatch correction in Escherichia coli.
Nucleic acids research - 11 Apr 1991
Aprelikova O, Jiricny J
Abstract excerpt
We wanted to establish whether strand breaks and gaps, arising during the removal of uracil from newly-synthesized DNA, can be utilized as strand discrimination signals by the methyl-directed mismatch repair system of Escherichia coli. For this purpose, we constructed a series of M13 heteroduplexes that contained a single uracil residue situated either upstream or downstream from a G/T or an A/C mispair....
Topics
- Amino Acid Sequence
- Base Composition
- Base Sequence
- DNA Repair
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Phenotype
- Transfection