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Article

A vaccinia virus isatin-beta-thiosemicarbazone resistance mutation maps in the viral gene encoding the 132-kDa subunit of RNA polymerase.

Virology - 1 Dec 1991

Condit R C, Easterly R, Pacha R F, Fathi Z, Meis R J

Abstract excerpt

The mutation in a vaccinia virus mutant resistant to inhibition by isatin-beta-thiosemicarbazone was mapped by marker rescue. DNA from the resistant mutant was cloned into cosmid and plasmid vectors and tested for its ability to convert wild-type vaccinia virus to IBT resistant virus in a helper-mediated marker rescue protocol. Resistance was mapped in this way to a 0.9-kb DNA fragment derived from the HindIII A...

Topics

Blotting, Southern
Cloning, Molecular
Cosmids
DNA Probes
DNA-Directed RNA Polymerases
Drug Resistance
Genes, Viral
Isatin
Mutation
Plasmids
Vaccinia virus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1962456
DOI: 10.1016/0042-6822(91)90559-t

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1962456-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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