Article
Functional expression of horsegram (Dolichos biflorus) Bowman-Birk inhibitor and its self-association.
Biochimica et biophysica acta - 1 Jul 2010
Muricken Deepa G, Gowda Lalitha R
Abstract excerpt
Horsegram (Dolichos biflorus), a protein-rich leguminous pulse, native to Southeast Asia and tropical Africa, contains multiple forms of Bowman-Birk inhibitors. The major Bowman-Birk inhibitor from horsegram (HGI-III) was cloned and functionally expressed in Escherichiacoli (rHGI), which moved as a dimer in solution similar to the natural inhibitor. The biochemical characterization of rHGI also points to its...
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