Article
In silico and in vitro analysis of a rational mutation in gIII signal peptide and its effects on periplasmic expression of rhGH in E. coli.
Archives of microbiology - 24 Aug 2022
Ghasemi Fahimeh, Zare Hamed, Zomorodipour Alireza, Shirzeyli Maryam Hosseinzade, Kieliszek Marek
Abstract excerpt
The secretion efficiency of a heterologous protein in E. coli is mainly dictated by the N-terminal signal peptide fused to the desired protein. In this study, we aimed to select and introduce mutations into the - 1, - 2 and - 3 positions of the gIII signal peptide (originated from filamentous phage fd Gene III) fused to the N-terminus of the human growth hormone (hGH), and study its effect on the secretion...
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