Article
Site-directed mutagenesis to identify key residues in structure-function relationship of winged bean chymotrypsin-trypsin inhibitor and 3-D structure prediction.
Protein and peptide letters - 1 May 2011
Roy Sanhita, Mandal Chhabinath, Dutta Samir K
Abstract excerpt
Winged bean chymotrypsin trypsin inhibitor (WbCTI) is a Kunitz type serine protease inhibitor that inhibits both trypsin and chymotrypsin at 1:1 molar ratio. Site-directed mutagenesis study was employed to generate two mutants of WbCTI, with an aim to explore its dual inhibitory properties against the proteases. The mutants were expressed in Escherichia coli and, were purified to homogeneity using a single step...
Topics
- Amino Acid Sequence
- Chromatography, Gel
- Chymotrypsin
- Escherichia coli
- Fabaceae
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plant Proteins
- Protein Binding
- Protein Structure, Tertiary
- Structure-Activity Relationship
- Trypsin Inhibitors