Article
Site-saturation mutagenesis of leucine 134 of Bacillus licheniformis nucleotide exchange factor GrpE reveals the importance of this residue to the co-chaperone activity.
The protein journal - 1 Jul 2010
Lin Min-Guan, Chen Bo-En, Liang Wan-Chi, Chou Wei-Mou, Chen Jiau-Hua, Kuo Lih-Ying, Lin Long-Liu
Abstract excerpt
To elucidate the role of leucine 134 of Bacillus licheniformis nucleotide exchange factor (BlGrpE), site-saturation mutagenesis was employed to generate all possible replacements for this residue. Wild-type and mutant proteins were purified by nickel-chelated chromatography and had a molecular mass of approximately 34.5 kDa. As compared with wild-type BlGrpE, the nucleotide exchange factor (NEF) activity of...
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