Article
The thermoduric effects of site-directed mutagenesis of proline and lysine on dextransucrase from Leuconostoc mesenteroides 0326.
International journal of biological macromolecules - 1 Feb 2018
Li Meng-Qi, Zhang Hong-Bin, Li Yao, Hu Xue-Qin, Yang Jing-Wen
Abstract excerpt
Dextransucrase (DSR, EC2.4.5.1) from Leuconostoc mesenteroides 0326 which catalyzes sucrose to produce dextran, are popularly used in food and medicine industries. However, its poor kinetic stability especially at higher temperatures is a limiting factor for developing industrial applications. The aim of this study is to improve the enzyme activity and thermal stability of dextransucrase by single, double and...
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