Article
Translational control assessed using the tethered function assay in Xenopus oocytes.
Methods (San Diego, Calif.) - 1 May 2010
Minshall Nicola, Allison Rachel, Marnef Aline, Wilczynska Anna, Standart Nancy
Abstract excerpt
The tethered function assay is a method designed to address the role of an RNA-binding protein upon the metabolism of a reporter RNA. The basis of this assay is to artificially tether a test protein to a reporter mRNA by employing an unrelated bacteriophage MS2 or lambda N RNA-protein interaction, and to assess the effects of the test protein on the reporter RNA. In this chapter, we first discuss the principles...
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