Article
Expression of glutathione S-transferase-cardiotoxin fusion protein in Escherichia coli.
Toxicon : official journal of the International Society on Toxinology - 1 Dec 1994
Chi L M, Vyas A A, Rule G S, Wu W G
Abstract excerpt
We report here the construction of cardiotoxin V gene, from cobra snake venom (Naja naja atra), by chemically synthesized oligonucleotides and its expression as a glutathione S-transferase-cardiotoxin fusion protein in the inclusion bodies of Escherichia coli. The expression of cardiotoxin fusion...
Topics
- Amino Acid Sequence
- Animals
- Cobra Cardiotoxin Proteins
- Elapid Venoms
- Escherichia coli
- Gene Expression Regulation, Enzymologic
- Glutathione Transferase
- Molecular Sequence Data
- Mutation
- Recombinant Fusion Proteins
- Structure-Activity Relationship
