Article
Expression of glutathione S-transferase-cardiotoxin fusion protein in Escherichia coli.
Toxicon : official journal of the International Society on Toxinology - 1 Dec 1994
Chi L M, Vyas A A, Rule G S, Wu W G
Abstract excerpt
We report here the construction of cardiotoxin V gene, from cobra snake venom (Naja naja atra), by chemically synthesized oligonucleotides and its expression as a glutathione S-transferase-cardiotoxin fusion protein in the inclusion bodies of Escherichia coli. The expression of cardiotoxin fusion protein in protein with a yield of about 35 mg/liter culture was confirmed by highly specific anti-peptide antibodies...
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