Article
Isolation and characterization of Escherichia coli mutants with altered rates of deletion formation.
Genetics - 1 Jan 1991
Whoriskey S K, Schofield M A, Miller J H
Abstract excerpt
Using site-specific mutagenesis in vitro we constructed a genetic system to detect mutants with altered rates of deletion formation between short repeated sequences in Escherichia coli. After in vivo mutagenesis with chemical mutagens and transposons, the system allowed the identification of mutants with either increased or decreased deletion frequencies. One mutational locus, termed mutR, that results in an...
Topics
- Base Sequence
- Blotting, Southern
- Chromosome Deletion
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Lac Operon
- Molecular Sequence Data