Article
Gamma-irradiated peripheral blood mononuclear cells can express LAK activity.
International journal of cell cloning - 1 Jan 1991
Chong A S, Bier D E, Grimes W J, Hersh E M
Abstract excerpt
Peripheral blood mononuclear cells (PBMC) irradiated with high dose gamma-radiation (1000-5000 rad) are commonly used as feeder cells during the cloning of T lymphocytes, natural killer (NK) and lymphokine activated killer (LAK) cells. We report here that such gamma-irradiated PBMC can be stimulated with interleukin 2 (IL-2) to express the ability to lyse a variety of tumor cell targets. The non-major...
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