Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression.

Abstract excerpt

Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, mediated independently through two cis-acting sites, termed the E and I silencers. We have found that in the absence of the I silencer, deletion of any one of three distinct elements within E yields partial derepression of the mating-type genes resident at HML, whereas...

Topics

• Chromosomes, Fungal
• Crosses, Genetic
• Gene Expression Regulation, Fungal
• Genes, Fungal
• Genes, Mating Type, Fungal
• Genotype
• Mating Factor
• Mutagenesis, Site-Directed
• Peptides
• Pheromones
• Restriction Mapping