Article
Residue 234 is a master switch of the alternative-substrate activity profile of human and rodent theta class glutathione transferase T1-1.
Biochimica et biophysica acta - 1 Apr 2010
Shokeer Abeer, Mannervik Bengt
Abstract excerpt
BACKGROUND: The Theta class glutathione transferase GST T1-1 is a ubiquitously occurring detoxication enzyme. The rat and mouse enzymes have high catalytic activities with numerous electrophilic compounds, but the homologous human GST T1-1 has comparatively low activity with the same substrates....
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Arginine
- DNA Primers
- Glutathione Transferase
- Humans
- Kinetics
- Mice
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Site-Directed
- Mutation
- Rats
- Substrate Specificity
- Tryptophan