Article
Diverging catalytic capacities and selectivity profiles with haloalkane substrates of chimeric alpha class glutathione transferases.
Protein engineering, design & selection : PEDS - 1 May 2008
Kurtovic Sanela, Shokeer Abeer, Mannervik Bengt
Abstract excerpt
Six homologous Alpha class glutathione transferases of human, bovine, and rat origins were hybridized by means of DNA shuffling. The chimeric mutants were compared with the parental enzymes in their activities with several alkyl iodides. In order to facilitate a multivariate analysis of relations...
Topics
- Alkanes
- Amino Acid Sequence
- Amino Acids
- Calorimetry
- Catalysis
- Glutathione Transferase
- Molecular Conformation
- Molecular Sequence Data
- Multivariate Analysis
- Mutation
- Protein Engineering
- Sequence Homology, Amino Acid
- Stereoisomerism
- Substrate Specificity