Article
Detection and biochemical characterisation of a novel polymorphism in the human GSTP1 gene.
Biochimica et biophysica acta - 1 Aug 2007
Kitteringham Neil R, Palmer Luke, Owen Andrew, Lian Lu-Yun, Jenkins Roz, Dowdall Sam, Gilmore Ian, Park B Kevin, Goldring Chris E
Abstract excerpt
The glutathione transferases (GSTs) mediate the detoxification of a broad spectrum of electrophilic chemicals. We report here the identification and characterisation of a novel naturally occurring transition that changes codon 169 from GGC (Gly) to GAC (Asp) in the human Pi class GST, GSTP1. Expression of the variant in human HepG2 cells led to a small increase in 1-chloro-2,4-dinitrobenzene (CDNB) conjugation...
Topics
- Alleles
- Amino Acid Substitution
- Aspartic Acid
- Carcinoma, Hepatocellular
- Cell Line, Tumor
- Circular Dichroism
- Dinitrochlorobenzene
- Escherichia coli
- Gene Frequency
- Genotype
- Glutathione S-Transferase pi
- Glutathione Transferase
- Humans