Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Solution-based scanning for single-base alterations using a double-stranded DNA binding dye and fluorescence-melting profiles.

The American journal of pathology - 1 Sept 2001

Elenitoba-Johnson K S, Bohling S D

Abstract excerpt

DNA molecules differing by as little as a single-base substitution have traditionally been distinguished by gel electrophoresis-based methodologies that exploit differences in the sequence-specific properties of double-stranded DNA (dsDNA) such as melting temperature and secondary conformational configuration. By comparison, solution-based fluorescence methods using sequence-specific probes are limited to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Coloring Agents
Share to Fluorescence
Share to Forecasting

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Techniques

Share to Nucleic Acid Denaturation

Share to Reference Values

Share to Solutions

Share to Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 11549577
DOI: 10.1016/S0002-9440(10)61760-9
PMCID: PMC1850450

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Solution-based scanning for single-base alterations using a double-stranded DNA binding dye and fluorescence-melting profiles.PMID 11549577Read ↗

DNA melting analysis for detection of single nucleotide polymorphisms.Semantic proximity Read ↗

High-resolution melting analysis of single nucleotide polymorphisms.Semantic proximity Read ↗

Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism.Semantic proximity Read ↗

HyBeacon probes: a new tool for DNA sequence detection and allele discrimination.Semantic proximity Read ↗

Optimized detection of sequence variation in heterozygous genomes using DNA microarrays with isothermal-melting probes.Semantic proximity Read ↗

Mutation detection using fluorescent hybridization probes and melting curve analysis.Semantic proximity Read ↗

Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes.Semantic proximity Read ↗

High-resolution melting analysis (HRMA): more than just sequence variant screening.Semantic proximity Read ↗

Identification of base pairs in single-nucleotide polymorphisms by MutS protein-mediated capillary electrophoresis.Semantic proximity Read ↗

Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis.Semantic proximity Read ↗

Masking selected sequence variation by incorporating mismatches into melting analysis probes.Semantic proximity Read ↗

Detection of simultaneous multi-mutations using base-quenched probe.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.