Article
Hyper-processive and slower DNA chain elongation catalysed by DNA polymerase III holoenzyme purified from the dnaE173 mutator mutant of Escherichia coli.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Apr 2002
Sugaya Yutaka, Ihara Keiichi, Masuda Yuji, Ohtsubo Eiichi, Maki Hisaji
Abstract excerpt
BACKGROUND: A strong mutator mutation, dnaE173, leads to a Glu612 --> Lys amino acid change in the alpha subunit of Escherichia coli DNA polymerase III (PolIII) holoenzyme and abolishes the proofreading function of the replicative enzyme without affecting the 3' --> 5' exonuclease activity of the epsilon subunit. The dnaE173 mutator is unique in its ability to induce sequence-substitution mutations, suggesting...
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