Development of stable, genetically well-defined conditionally viable Escherichia coli strains.

doi:10.1007/BF00260705

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Article

Development of stable, genetically well-defined conditionally viable Escherichia coli strains.

Molecular & general genetics : MGG - 1 May 1991

Degryse E

Abstract excerpt

The diaminopimelate (DAP) pathway provides the cell with lysine and with DAP, a vital cell wall constituent. Mutations in the DAP pathway of lysine biosynthesis are lethal for cells exposed to lysine in the absence of DAP. In this paper, the substitution of the dapD gene of Escherichia coli with...

Topics

Cloning, Molecular
DNA Transposable Elements
Diaminopimelic Acid
Escherichia coli
Genes, Bacterial
Genotype
Kanamycin Resistance
Mutation
Plasmids
Restriction Mapping

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