Differential regulation of two genes encoding lysyl-tRNA synthetases in Escherichia coli: lysU-constitutive mutations compensate for a lysS null mutation.

Abstract excerpt

Lysyl-tRNA synthetases are synthesized in Escherichia coli from two distinct genes, lysS and lysU, which are regulated differentially. A strain which is null for lysS, the constitutive gene, was created by gene disruption (lysS1) and exhibited cold-sensitive lethality. Hence, lysS is dispensable at high temperatures. This cold sensitivity was suppressed by a multi-copy plasmid carrying lysU, the inducible gene....

Topics

• Base Sequence
• Cell Division
• DNA Transposable Elements
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Lysine-tRNA Ligase
• Molecular Sequence Data
• Mutation
• Promoter Regions, Genetic
• Regulatory Sequences, Nucleic Acid