Energy transfer in lactose repressor protein modified with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonate.

doi:10.1021/bi00224a019

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Article

Energy transfer in lactose repressor protein modified with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonate.

Biochemistry - 12 Mar 1991

Gardner J A, Matthews K S

Abstract excerpt

Energy transfer between the two tryptophan residues in the lactose repressor protein and the fluorescent moiety of the cysteine-specific reagent N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonate (1,5-IAEDANS) has been examined. Modification of repressor with this compound did not affect op...

Topics

Chromatography, High Pressure Liquid
Energy Transfer
Lactose
Mutation
Naphthalenesulfonates
Peptide Mapping
Repressor Proteins
Spectrometry, Fluorescence

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