Article
Fluorescence quenching studies of Trp repressor using single-tryptophan mutants.
Journal of protein chemistry - 1 Nov 1995
Blicharska Z, Wasylewski Z
Abstract excerpt
Time-resolved and steady-state fluorescence have been used to resolve the heterogeneous emission of single-tryptophan-containing mutants of Trp repressors W19F and W99F into components. Using iodide as the quencher, the fluorescence-quenching-resolved spectra (FQRS) have been obtained The FQRS method shows that the fluorescence emission of Trp99 can be resolved into two component spectra characterized by maxima...
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