Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Crystal structures of spin labeled T4 lysozyme mutants: implications for the interpretation of EPR spectra in terms of structure.

Biochemistry - 25 Jul 2000

Langen R, Oh K J, Cascio D, Hubbell W L

Abstract excerpt

High resolution (1.43-1.8 A) crystal structures and the corresponding electron paramagnetic resonance (EPR) spectra were determined for T4 lysozyme derivatives with a disulfide-linked nitroxide side chain [-CH(2)-S-S-CH(2)-(3-[2,2,5,5-tetramethyl pyrroline-1-oxyl]) identical with R1] substituted at solvent-exposed helix surface sites (Lys65, Arg80, Arg119) or a tertiary contact site (Val75). In each case,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophage T4
Share to Binding Sites
Share to Crystallography, X-Ray

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Electron Spin Resonance Spectroscopy

Share to Muramidase

Share to Protein Conformation

Share to Spin Labels

Share to Static Electricity

Source

Identifiers

PMID: 10913245
DOI: 10.1021/bi000604f

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Crystal structures of spin labeled T4 lysozyme mutants: implications for the interpretation of EPR spectra in terms of structure.PMID 10913245Read ↗

Site-directed spin labeling of a genetically encoded unnatural amino acid.Semantic proximity Read ↗

High-resolution structure of a protein spin-label in a solvent-exposed β-sheet and comparison with DEER spectroscopy.Semantic proximity Read ↗

Characterisation of disulfide-bond dynamics in non-native states of lysozyme and its disulfide deletion mutants by NMR.Semantic proximity Read ↗

Structural characterization of the Cu(II)-NTA spin label on α-helices by X-ray crystallography and electron paramagnetic resonance.Semantic proximity Read ↗

Structural variability of E. coli thioredoxin captured in the crystal structures of single-point mutants.Semantic proximity Read ↗

Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): a study of doubly-spin-labeled T4 lysozyme.Semantic proximity Read ↗

Structural insights into the catalytic mechanism of Trypanosoma cruzi GPXI (glutathione peroxidase-like enzyme I).Semantic proximity Read ↗

NMR studies of structure, hydrogen exchange, and main-chain dynamics in a disrupted-core mutant of thioredoxin.Semantic proximity Read ↗

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure.Semantic proximity Read ↗

Lessons from the lysozyme of phage T4.Semantic proximity Read ↗

NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.Semantic proximity Read ↗

The role of cysteine oxidation in the thermal inactivation of T4 lysozyme.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.