Article
Hepatocellular phenotype in vitro is influenced by biophysical features of the collagenous substratum.
Hepatology (Baltimore, Md.) - 1 Feb 1991
Lindblad W J, Schuetz E G, Redford K S, Guzelian P S
Abstract excerpt
Hepatocytes maintained on different substrata in vitro possess strikingly different morphological and biochemical features. Rounded, multicellular aggregates of hepatocytes are seen if the cells are plated onto Matrigel, a reconstituted basement membrane, whereas a flattened, monolayer of hepatocytes is observed with Vitrogen. Hepatocellular protein synthesis is much greater on the Matrigel, although collagen...
Topics
- Actins
- Animals
- Cells, Cultured
- Collagen
- Cross-Linking Reagents
- Culture Media
- Cytochrome P-450 Enzyme System
- Liver
- Male
- Phenobarbital
- Phenotype
- Protein Biosynthesis