Article
Monolayer and spheroid culture of human liver hepatocellular carcinoma cell line cells demonstrate distinct global gene expression patterns and functional phenotypes.
Tissue engineering. Part A - 1 Mar 2009
Chang Tammy T, Hughes-Fulford Millie
Abstract excerpt
Understanding cell biology of three-dimensional (3D) biological structures is important for more complete appreciation of in vivo tissue function and advancing ex vivo organ engineering efforts. To elucidate how 3D structure may affect hepatocyte cellular responses, we compared global gene expression of human liver hepatocellular carcinoma cell line (HepG2) cells cultured as monolayers on tissue culture dishes...
Topics
- Actins
- Carcinoma, Hepatocellular
- Cell Proliferation
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Genes, Neoplasm
- Humans
- Liver
- Liver Neoplasms
- Phenotype
- Reverse Transcriptase Polymerase Chain Reaction
- Spheroids, Cellular
- Tumor Cells, Cultured