Article
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
Toxicological sciences : an official journal of the Society of Toxicology - 1 Jun 2007
Page Jeanine L, Johnson Mary C, Olsavsky Katy M, Strom Steven C, Zarbl Helmut, Omiecinski Curtis J
Abstract excerpt
Previously, we demonstrated that primary cultures of rat hepatocytes evidence higher levels of differentiated function when cultured in the presence of a dilute overlay of extracellular matrix (Matrigel). In this investigation, we used DNA microarrays, quantitative RT-PCR, immunoblotting, and cell morphology analyses to evaluate the biological responses imparted by Matrigel overlays on primary cultures of human...
Topics
- Blotting, Western
- Cell Differentiation
- Cells, Cultured
- Cluster Analysis
- Collagen
- DNA, Complementary
- Drug Combinations
- Environment
- Extracellular Matrix
- Focal Adhesions
- Gene Expression Profiling
- Gene Expression Regulation
- Hepatocytes