Article
Interaction of the glucocorticoid receptor DNA-binding domain with DNA as a dimer is mediated by a short segment of five amino acids.
The Journal of biological chemistry - 15 Feb 1991
Dahlman-Wright K, Wright A, Gustafsson J A, Carlstedt-Duke J
Abstract excerpt
We have previously shown that protein-protein interactions mediate cooperative binding of the glucocorticoid receptor DNA-binding domain to a glucocorticoid response element (Dahlman-Wright, K., Siltala-Roos, H., Carlstedt-Duke, J., and Gustafsson, J.-A. (1990) J. Biol. Chem. 265, 14030-14035). The cooperativity of DNA binding is lost when the distance between the two half-sites constituting a glucocorticoid...
Topics
- Amino Acid Sequence
- DNA
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Glucocorticoids
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Receptors, Glucocorticoid