Article
DNA binding specificity of mutant glucocorticoid receptor DNA-binding domains.
The Journal of biological chemistry - 15 Feb 1991
Zilliacus J, Dahlman-Wright K, Wright A, Gustafsson J A, Carlstedt-Duke J
Abstract excerpt
Mutation of a small number of amino acids in the DNA-binding domain of the estrogen receptor to the corresponding sequence of the glucocorticoid receptor switches the specificity of the receptor in transactivation assays (Mader, S., Kumar, V., de Verneuil, H., and Chambon, P. (1989) Nature 338, 271-274). We have made the corresponding reciprocal mutations in the context of the glucocorticoid receptor DNA-binding...
Topics
- Amino Acid Sequence
- DNA
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Receptors, Glucocorticoid
- Substrate Specificity
- Transcription, Genetic