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Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010

Zhang Xiao-Qing, Zhang Su-Chun

Abstract excerpt

Directed differentiation of human embryonic stem cells (hESCs) to a functional cell type, including neurons, is the foundation for application of hESCs. We describe here a reproducible, chemically defined protocol that allows directed differentiation of hESCs to nearly pure neuroectodermal cells and neurons. First, hESC colonies are detached from mouse fibroblast feeder layers and form aggregates to initiate the...

Topics

Animals
Cell Aggregation
Cell Differentiation
Coculture Techniques
Culture Media, Serum-Free
Dopamine
Embryonic Stem Cells
Humans
Mesencephalon
Mice
Neurogenesis

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Identifiers

PMID: 19907987
DOI: 10.1007/978-1-60761-369-5_19
PMCID: PMC2948208

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