In vitro neural differentiation of human embryonic stem cells using a low-density mouse embryonic fibroblast feeder protocol.

doi:10.1007/978-1-60761-369-5_4

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Article

In vitro neural differentiation of human embryonic stem cells using a low-density mouse embryonic fibroblast feeder protocol.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010

Ozolek John A, Jane Esther P, Esplen James E, Petrosko Patti, Wehn Amy K, Erb Teresa M, Mucko Sara E, Cote Lyn C, Sammak Paul J

Abstract excerpt

Human embryonic stem cells (hESCs) have the capacity to self-renew and to differentiate into all components of the embryonic germ layers (ectoderm, mesoderm, endoderm) and subsequently all cell types that comprise human tissues. HESCs can potentially provide an extraordinary source of cells for tissue engineering and great insight into early embryonic development. Much attention has been given to the possibility...

Topics

Animals
Biomarkers
Cell Count
Cell Differentiation
Cell Line
Coculture Techniques
Cryopreservation
Embryonic Stem Cells
Fibroblasts
Humans
Immunohistochemistry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mice

Neurogenesis

Neuroglia

Neurons

Phenotype

Pluripotent Stem Cells

Source

Open in PubMed

Identifiers

PMID: 19907972
DOI: 10.1007/978-1-60761-369-5_4

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