Article
Sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens.
The Journal of general virology - 1 Mar 2010
Cunningham Charles, Gatherer Derek, Hilfrich Birgitta, Baluchova Katarina, Dargan Derrick J, Thomson Marian, Griffiths Paul D, Wilkinson Gavin W G, Schulz Thomas F, Davison Andrew J
Abstract excerpt
We have assessed two approaches to sequencing complete human cytomegalovirus (HCMV) genomes (236 kbp) in DNA extracted from infected cell cultures (strains 3157, HAN13, HAN20 and HAN38) or clinical specimens (strains JP and 3301). The first approach involved amplifying genomes from the DNA samples as overlapping PCR products, sequencing these by the Sanger method, acquiring reads from a capillary instrument and...
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