Article
A method enabling high-throughput sequencing of human cytomegalovirus complete genomes from clinical isolates.
PloS one - 1 Jan 2014
Sijmons Steven, Thys Kim, Corthout Michaël, Van Damme Ellen, Van Loock Marnix, Bollen Stefanie, Baguet Sylvie, Aerssens Jeroen, Van Ranst Marc, Maes Piet
Abstract excerpt
Human cytomegalovirus (HCMV) is a ubiquitous virus that can cause serious sequelae in immunocompromised patients and in the developing fetus. The coding capacity of the 235 kbp genome is still incompletely understood, and there is a pressing need to characterize genomic contents in clinical isolates. In this study, a procedure for the high-throughput generation of full genome consensus sequences from clinical...
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