Article
"cAMP sponge": a buffer for cyclic adenosine 3', 5'-monophosphate.
PloS one - 3 Nov 2009
Lefkimmiatis Konstantinos, Moyer Mary Pat, Curci Silvana, Hofer Aldebaran M
Abstract excerpt
BACKGROUND: While intracellular buffers are widely used to study calcium signaling, no such tool exists for the other major second messenger, cyclic AMP (cAMP). METHODS/PRINCIPAL FINDINGS: Here we describe a genetically encoded buffer for cAMP based on the high-affinity cAMP-binding carboxy-terminus of the regulatory subunit RIbeta of protein kinase A (PKA). Addition of targeting sequences permitted localization...
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