Article
Determination of the 5'-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells.
Archives of virology - 1 Jan 2009
Ichiyama Koji, Yamada Kentaro, Tanaka Toshinori, Nagashima Shigeo, Jirintai, Takahashi Masaharu, Okamoto Hiroaki
Abstract excerpt
Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5'-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the genotype 3...
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