Article
Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray.
Antimicrobial agents and chemotherapy - 1 Jan 2010
Coyne Sébastien, Guigon Ghislaine, Courvalin Patrice, Périchon Bruno
Abstract excerpt
An oligonucleotide-based DNA microarray was developed to evaluate expression of genes for efflux pumps in Acinetobacter baumannii and to detect acquired antibiotic resistance determinants. The microarray contained probes for 205 genes, including those for 47 efflux systems, 55 resistance determinants, and 35 housekeeping genes. The microarray was validated by comparative analysis of mutants overexpressing or...
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