Article
Microdroplet-based PCR enrichment for large-scale targeted sequencing.
Nature biotechnology - 1 Nov 2009
Tewhey Ryan, Warner Jason B, Nakano Masakazu, Libby Brian, Medkova Martina, David Patricia H, Kotsopoulos Steve K, Samuels Michael L, Hutchison J Brian, Larson Jonathan W, Topol Eric J, Weiner Michael P, Harismendy Olivier, Olson Jeff, Link Darren R, Frazer Kelly A
Abstract excerpt
Targeted enrichment of specific loci of the human genome is a promising approach to enable sequencing-based studies of genetic variation in large populations. Here we describe an enrichment approach based on microdroplet PCR, which enables 1.5 million amplifications in parallel. We sequenced six samples enriched by microdroplet or traditional singleplex PCR using primers targeting 435 exons of 47 genes. Both...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.